Immulite 2000 Online Training Flashcards
main top covering parts of immulite 2000
reagent carousel cover, bead carousel cover, sample carousel door, DRD primimg or substrate reservoir door and monitor arm
main interior parts of immulite 2000
reagent carousel, sample carousel, bead carousel, DRD ( dual resolution dilution), pipettors, dilution well, pumps, tube processor
consumable parts of immulite 2000
solid waste container, liquid waste container, tube hopper, water bootle, probe wash bottle, substrate reservoir
solid waste is used to
collect used reaction tubes after they are read by PMT
tube hopper is used to
hold reaction tubes
probe wash is used to wash
sample and reagent pippetors
liquid waste is used to
collect waste fluid from pippetor wash well, dilution well, tube/bead wash
bead is
specific part for any test that antibody has been coated to it
water is at wash station to remove
any residual unbound reagent and sample
software screen on immulite 2000 is including
home screen, bead/reagent screen/ help screen/ LIS screen
home screen has find button that is used for
searching accession number of test and seeing tube location
home screen has beads button for
checking bead pack
reagent carousel on home screen is used for
checking wedge reagents
consumable indicators is used to check levels of
water, substrate, probe wash, tube hopper, liquid and solid waste
in sample rack on home screen you can see
accession number of tubes in sample rack
home screen also has
horizontal toolbar and vertical toolbar
DRD function is to
move sample and reagent to the pepittors
during analysis opening DRD priming/ substrate reservoir door
does not bring the system to pause mode
mentor means
...
reagent carousel has
24 positions
ordering adjuster procedure can be found on
help screen
for loading and unloadning samples into sample carousel we have to
pause the system first and after that put the system back into run mode
when system is in pause mode
the letters on the pause buttopn are red
if system is not connected to LIS we have to
order manually after starting run mode
microsample tube is used when the volume is
<250 microliter
for loading microtube we use
microtube holder
the dead volume of microsample tube is
50 microlitter
it is recommended for microsample no
more than 5 tests
microsample tube holders are
reuseable and have permanent barcode label
for adding test to sample that is in progress we go
to worklist and press new and type accession number and seledct the test
in the result view screen
results for one sample are seen
we have 2 type of dilution on immulite 2000
onboard dilution and manual dilution
for onboard dilution we put diluent in
100 X 16 mm tube
for manual dilution deluent should be diluted with
1 part deluent and 1.5 par water
a square tube position on the sample rack indicates
micro sample tube
the components of IMMULITE kit are
IFU, kit barcode, reagent wedge, bead pack, adjusters, important notice
the kit barcode should be scanned with hand-held scanner for
loading reagent wedge and bead pack
tilt down means
...
we have to scan kit barcode when we want to run a
new lot number
the barcode labels of reagent wedge and bead pack should be
toward outside
adjuster can be in either liquid or
lyophilized
barcode label of kit located in
box flap
IFU means
information for use
packege insert is same as
IFU or instruction
before using a new kit
adjustment must be run
adjustment corrects variation between
manufactur's system and our system
storing and handling of adjusters are critical for
achieving accurate results
when we have adjustors results we have to decide adjustment is valid or not based on
adjustment validation guidline
adjustment validation guideline has 3 steps
evaluating QC, evaluating slop, evaluating intercept
glide of kit
...
for each level of adjusters instrument does
4 replicates
if there is a high intercept for sandwich assay low end level of QC
most likely affected
for preparation of reagent wedge to be loaded we should
use a lint-free tissue to dry the glide and the areas surrounding it
controls are running to verify
adjustment validity and verify reagent and beads viability
for entering new QC data we have to go to
control entry screen
for accessing control entry screen
select the QC from horizontal bar and then data entry
for entering new QC information we should insert
Q C name, QC lot number< QC level, add new test< mean SD and SD multiplier equal to 2 and press calculate range
when we enter new control information accession number
automatically filled by software
grid means
...
deflector means
...
when put solid waste in the location we have to make sure
oroperly place the deflector
logging off the system initiate
the system back up
weekly maintenance consists of
waste tube cleaning diagnostic
cleaning waste tube on weekly maintenance prevents
bacterial growth and facilitate effective system performance
monthlu meintenace consists of
checking dispense angle regent probe, performing water test pm, cleaning the fan filter, decontaminating clot detection transducer, decontaminating the lines and bottles
checking dispense reagent probe angle veifies
the integrity of reagent probe for effective aspiration and dispense
the water test pm is used to check
water on the system for alkaline phosphatase contamination that
cleaning the fan filter on a monthly basis prevents
overheating of computer and hard dfrives
the quarterly maintenance of immulite consists of
replacement of CO2 scrubber
0.1 NAOH is used for
decontamination of transducer
probe cleaning solution is used also for
waste tube cleaning
condense means
...
if probe enters to gel barrier of the tube we have to
replace the probe
after adding substrate to reaction tube
luminometer incubation happens
Allergy assay on immulite 2000 measures
the amount of immunoglobulin (IgE) to a specific allergen in patient serum