Phlebotomy Flashcards ionicons-v5-c

Tenderly to automated skin incision device

That is used for make a skin puncture in the finger or heel for the collection of capillary blood used for microhematocrit measurement test per formed on capillary bloodm

Cytogenetic analysis

Requires whole blood collected in a green topped / Na heparin blood collection tube

Red topped tubes

Contain no additive but sodium heparin is required for chromosomes analysis

Gray topped tubes

Contain potassium oxalate and sodium flouride or lithium iodoacetate and heparin which will interfere in chromosome analysis

Light blue topped tubes

Contain sodium citrate which will interfere in chromosome analysis

Microscopy specimens

Are usually urine collections and therefore do not require a blood collection system

Clinical chemistry specimens

Do not require culture vials

Clinical immunology specimens

Do not require culture media vials

BD bactec culture vials

Are used for microbiology collections

Erythrocytes sedimentation rate

Requires a black topped tube with sodium citrate or purple top tube with edta

Zinc levels maybe erroneous

If collected with lithium heparin since the green top tubes are not manufactured for trace element collection

Lithium heparin

Is found in green top tubes and is very suitable for blood glucose measurements

The blood lithium level

Wi be falsely increased if collected with lithium heparin anticoagulant

Royal blue top tubes

Generally contain sodium heparin, edta or no anticoagulant

Sodium citrate

Is generally used in light blue top tubes

Ammonium heparin is not

Used in blood collection tubes

Tripod I'm citrate is

Normally used in light blue top tubes

Sodium polynethole sulfonate/ sps

Is used in the collection of blood culture specimens

Heparinized whole blood

Has become the specimen of choice for the clinical chemistry instruments used in STAT situations

BD vacutainer systems

Is a collection and dilution unit used for various procedures WBC, RBC, platelet, hemoglobin, RBC fragility and sodium, potassium and lead determination

Heparin

Prevents coagulation by in activating blood clotting thrombin and thromboplastin

Sodium flouride

Is an additive to inhibit glycolytic action/ glucose breakdown.

Coagulation of blood

Can be prevented of oxalates, citrates and or edta by their ability to remove calcium, forming insoluble calcium salts

The smaller the gauge number

The larger the diameter of the needle

Tenderletts breakdown safe

Single use, atomically retracting, disposable devices used for microcollection by skin puncture

Green top tubes

Contain heparin that can alter the accuracy of blood counts

Purple top tubes

Blood cell count, WBC, RBC, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, requires whole blood collected in purple tube

Gray top tubes

Contain sodium flouride and pottasium oxalate or lithium iodoacetate and heparin. These additives can falsely alter the blood cell count levels.

The APTT/ activated partial thromboplastin

Requires blood collection in the light blue top tubes containing citrate

The RPR tests

Is a syphilis test requiring serum collected I'm red or speckled top tubes

The VDRL test

Is used to detect syphilis in blood and requires a red or speckled top tube for collection

Selenium

Is a trace element and must be tested on blood collected in a trace element tube, royal blue with heparin or in edta purple tube

Cortisol

Is a hormone and to measure it, blood needs to be collected in a green top tube

CBC levels

Requires blood collected in edta/ purple top tube

The enzyme, lactate dehydrogenase

Needs to be detected in a blood sample collected with speckled top tube

Lead levels in blood are very minute and

Require a blood collection tube without any detectable lead such as the brown top tube

The BD microtainer tube

Is frequently used in the microllection of electrolytes and general chemistry blood specimens

Microvette capillary blood collection system

Is used to collect and store capillary blood specimens

The BD unopette

Is a microcollection and dilution device that is used to measure various analytes

Green top tube

Contains heparin which is not a glycolytic inhibitor

Gray top tube

Contains a glycolytic inhibitor used in glucose testing

Gray top tube

Contains sodium flouride that destroys alkaline phosphatase and many other enzymes in the blood.

Speckled top tube

Can be used for the collection of alkaline phosphatase and many other enzymes in the blood

BD unopette

Is a blood collection device that is prefilled with specific amounts of diluent or reagents

Vacuette tube

Is a blood collection tube that does not have a diluent for dilution of the blood

Heparinized microcollection tubes

Have an anticoagulant but are not prefilled with a dilution of the blood.

The safety T fill capillary blood collection device

Does not have solutions for dilution of the blood

Surgicutt

Is a sterile standarized, disposable instrument that is used for bleeding time assay

Tenderletts

Is a safety microcollection device

BD unopette

Is a blood collection and dilution device used in the measurement of the RBC count, WBC count, platelet count and other procedures but not bleeding time

The gauge size

Of the needle is identified by the color code on the sealed shiekd

Lead

Is an analyte that must be collected in a brown top vacuum tube, but it does not have to be protected from light

Bilirubin

Breaks down chemically when exposed to light and thus the blood must be protected from light bili rub is to be measured

Citrate phosphate dextrose/CPD

Is an anticoagulant and preservative that is frequently used in the collection of blood for blood donations

Potassium oxalate

Is an anticoagulant that is used in the gray too vacuum tubes for blood glucose testing

Acid citrate dextrose/ACD

Is an anticoagulant and preservative that is frequently used in the collection of blood for blood donations

Sodium citrate

The anticoagulant in light blue top blood collection tubes is frequently used in coagulation blood studies

Hepanized microcollection tube

Is a microcollection capillary tube that contains heparin to mix with the blood to avoid blood coagulation

The microhematocrit tube

Is a microcollection capillary tube that contains heparin to mix with the blood to avoid blood coagulation

BD Vacutainer serum separator tube

Contains a separator barrier to separate the blood cells from the serum

Hemolysis

Is the breakdown of RBC which is not the most critical reason for separating blood cells from the serum

Glycolysis

Is the break down of glucos, which can lead to erroneous low blood glucose results if the RBC are not separated from the serum

Blood for creatinine kinase

Can be collected in the red top tube since it contains no interfering additives

Blood for creatine kinase can be collected

In the speckled top tube since it separates serum from the blood cells and has no effect on the creatine kinase level

Blood for creatine kinase

Can be collected in the gold top tube since it contains no interfering additives

The external tube diameter and light plus the maximum amount

Of specimen to be collected into the vacuum tube are the criteria that are used to describe vacuum collection tube size

The evacuated tube system

Requires three components, the evacuated sample tube, the double pointed needle and a special plastic adapter

If a light blue top tube

Is underfilled coagulation results will be erroneous prolonged

Potassium value is

Determined from the blood collected in the green top tube.

Pt and ptt tests

Are performed on blood collected with the anticoagulant sodium citrates. If the require blood level is not collected in the sodium citrate tube, then the ration of sodium citrate to blood is increase causing erroneous prolonged coagulation results

Tan top tube

Has been designed for the collection of lead determination s

Royal blue top tube

Is designed for collection of trace such as copper

Purple top tube

Contains anticoagulant edta and is generally used for hematology testing

Ethylene diamine tetra acetic acid/ Edta

Is used to collect blood for blood smears since it creates minimal distortion of WBC

The volume of cumulative blood to be collected during a hospital stay

1 month or less from a new join weighing 6 to I lbs is approximately 23 ml

The red and black speckled top blood a collection tube

Should be gently inverted five times so that the blood clotting occurs in 30 minutes

Creatine kinase testing

Is a chemistry test and should be occur in the red and black speckled top tube that yields blood cells and serun

ESR

Is the same laboratory assay as a sedimentation rate, erythrocyte sedimentation rate or red rate

The royal blue top tube has been

Designed to collect sterile blood specimens for trace elements, toxicology and or nutritional studies

Dinning/ putting on a clean pair of gloves in the presence of the patient

Provides a reassuring and safety conscious gesture for the patient and the health care worker

MRSA signifies

Methicillin resistant staphylococcus aureus

Laboratory requisition for clinical testing

Contains patients full name, identification number and date of virth, the dates and types of tests to be performed, the physicians name, the location of the patient and timing instructions

Hand lotions are used to

Relieve dryness caused by excessive hand washing or excessive used of hand rubs. They minimize the occurrence of dermatitis associated with hand decontamination

Alcohol based hand rubs are

Effective for hand decontamination purposes if the hands are not visibly soiled

Phlebotomist should follow the manufacturer

Recommended amouny of hand rub to decontaminate hands. Usually it is a small s mount that should be applied on one palm, then while rubbing hands together.. All surfaces of hands and fingers should be covered and rubbed until hands are dry

When arm veins cannot be used for venipuncture

The preferred site is that back of the hand or wrist that is the dorsal surface not the palm side of the hand

A tourniquet should be applied

About 3 inches above the venipuncture site. It should be tight but not painful and should be partially looped to allow for easy release with one hand

A tourniquet causes

Pooling or filling of blood in veijs. Such pooling makes blood collection easier during a venipuncture procedure, regardless of the method used to withdraw blood from the vein

Alcohol should be allowed to

Air dry for 30 to 60 seconds, this prevents re contamination, prevents stinging and prevents the chances of contaminating the specimen with the alcohol

The needle should be

Inserted in the same direction as the veins at a 15 to 30 degree angle to the skin

After a needle is inserted and the blood begins to flow

The tourniquet should be released and the patient may open his or her fist

Blood culture tubes

Yellow top tubes are always collected first to reduce the chances of contamination

The coagulation tube

Light blue top tubes should be collected before the hematology tube or purple top tubes

All specimens labels should include the

Patients name and identification number, the date and time of collection and the phlebotomist initials. The patients room number, bed assignment, or out patient status is optional but useful information

Povidone iodine preparation are used primarily for

Decontaminating the skin before venipuncture for blood gas analysis and blood cultures. Excess iodine should be removed from the skin with sterile gauze before the puncture

Many laboratory tests can be affected by the

Ingestion of food and drink. It is important to note whether or not the patient has been fasting

To increase the likelihood of a successful puncture

Slight rotation of the patients arm to a different position, palpation the entire antecubital area to trace vein path, warming the site, gentle massaging, lowering the arm over the bed side, asking the patient for a preferred site, or using the other arm. But ask the doctor for a preferred site.

For hand vein punctures

The posterior , dorsal surface of the wrist is preferred because the veins are larger.

The patients who have mastectomies

Often have lymph nodes removed from the same surgical area. Swelling often occurs on the affected side of the body. Blood should not be withdrawn from the mastectomy side unless approved by a physician. The other arm should be considered first as an alternate site

In a venipuncture procedure

The needle should always be inserted with the bevel side upward and directly above a prominent vein or slightly below the palpable vein.

Wing infusion sets are used for difficult venipuncture

Patients with small hand and wrist veins, patients who are young children, severely burned, geriatric, patients who have numerous needlestick/ oncology patients, restrictive position/ arthritis and fragile skin. Young and adult males

Peak and trough levels

Of certain drugs are useful to monitor the therapeutic levels.

The preferred order of draw would be

Blood cultures, coagukation, hematology and chemistry tubes. However, phlebotomist must follow the order of draw recommended by individual manufacturers and by their health care organizations

A butterfly system could be used to diminish the likelihood of

Complications, in addition the phlebotomist should be careful with site selection and document the patients conditions to explain ung results

bioethics refers to

life and death issues

cross examination is

used during the trial to obtain information regarding the possibility of malpractice of a health care worker or in other type of trials as well

the best definition for ethics is

moral standards of behaviour

an IRB requires

a blood collector in research project to attend a course on the protection of research participants

employer is legally responsible for monitoring HIV post exposure follow up

the CLIA88 was passed to

ensure that quality and accuracy of lab testing

violation of patients confidiantially

can be considered professional negligence

the judicial brach of the govt

establishes case law

what is cross examination in malpractice lawsuit

information obtained during the trial to obtain information regarding the possibilty of malpractice of a health care worker

The clinical laboratory contributes to all of the following except:

decontaminating the surgery rooms with disinfectants to kill amebic organisms

A nosocomial infection occurs when:

the chain of infection is complete

Disinfectants are:

chemicals that are used to remove or kill pathogenic microorganisms

If an accident occurs, such as a needlestick, the injured health care provider should immediately:

cleanse the area with isopropyl alcohol and apply and adhesive bandage

The skin puncture site should be

Cleaned with 70% isopranol and thoroughly dried before being punctured because residual alcohol prevents round drops of blood from forming which is needed for blood smear preparation

Residual alcohol may

In fact provide an extra measure of sterility, it does not provide an extra measure of test reliability

The finger stick procedure involves

Holding a patient's finger firmly with the phlebotomist thumb away from the puncture site. The puncture should be done on one sharp, continuous movement and gentle pressure can be applied to the finger the preferred site for skin puncture are the third and fourth fingerm

The preferred site is

The fleshy, central surface of the fingertip, not the extreme tip on. On the third finger

Capillary blood acquired by skin puncture

Is significantly different than that of venous blood acquired by venipuncture. It is composed of blood from arteries, venules, capillaries and intracellular and interstitial fluids

Synovial fluid is

Fluid found in between joints.

Interstitial fluid

Is a tissue fluid

Using the first drop of blood

Can have an adverse on a skin puncture specimen because it may be diluted with tissue fluids thus causing erroneous results in laboratory test

Good blood smears

Should cover about one half of the slide, have feathered edge

Blood smears

No rideges, lines, or holes should be present on a blood smear

Skin puncture blood is composed of

Tissue fluids, blood from arterioles, and venules and blood from capillaries. The content of arterial blood is actually grater in skin puncture blood than venous blood because the arterial pressure in the capital is stronger than the venous pressure

Skin puncture samples are often used for

White blood cell differentials.they are also used for a variety of screening tests. E.g glucise, cholesterol. And other point of care procedures

Blood gases are usually

Evaluated using arterial samples

Drug levels

Usually requires urine or larger amounts of blood than are possible from a skin puncture

Warming a site skin

For skin puncture increases blood flow to the site

The best angle for spreading

A blood smear using two glass slides is approximately 30 degress

The average depth of a skin puncture

Should be 2 to 3 mm for an adult to avoid hitting the bone

Osteomyelitis

Is defined an inflammation and infection of the bone

A capillary tube fills with blood during a puncture procedure using capillary action

Whereby blood flows freely into the tube on contact without suction

Cell morphology

May change because of inadequate filling, thereby altering the blood to additive ration

Fasting status

Is important for collecting many types of blood specimens

The fingers of newborns

Are not recommended for skin punctures sites

Laser devices

Are skin puncture alternatives

The order of draw for filling microcollection tubes

With capillary blood is noy the same as for venipuncture

Cyanotic finger is

Bluish in color due to insufficient oxygen

During the skin puncture procedure

The cut should be oriented across the fingerprint lines

Contaminated gloves

Should be disposed of in a biohazardous container adjacent to the procedure area

why is it important for phle to enjoy communicating with patients

improve the likelihood of effective quality care

when a phle builds a good rapport with a patient,what is he or she doing

being courteous and showing interest to improve patient satisfaction

which of the following is the appropriate protocol for health care worker who are involved in specimen collection

the blood collector should state that the patients physician ordered blood to be collected for testing and that it would be best to discuss the lab tests with the physician

braille is defined as which of the following

writing system for sightless individuals

why is a documentation of all clinical events important

to monitor quality and coordination of care

what is an RFID

identification tag using silicon chips and a wireless receiver

a critical value is a

test result that may be life threatening

what should a phleb do if there are too many visitors in a patient room when a blood specimen is to be collected STAT

politely ask the visitors to step into the hall while the blood is being collected

The value of leach therapy lies in the components of the worm's_____.

Saliva; which contains a local vasodilator, a local anethetic, and hirudin (an anticoagulant)

Polycythemia

a disorder involving the overproduction of red blood cells.

Certification

A voluntary process by which an agency grants recognition to an individual who has met certain prerequisites in a particular technical area.

Licensure

an official document or permit granted by a state agency that gives legal permission for a person to work in a particular health profession.

Continuing Education

Designed to update the knowledge or skills of participants and is generally geared to a learning activity or course of study for a specific group of health professionals, such as phlebotomists.

By recognizing diversity

The phlebotomist promotes goodwill and harmonious relationships that directly improve health outcomes, the quality of services, and public relations.

Professionalism

Is defined as the conduct and qualities that characterize a professional person.

Self confidence

Phlebotomists who exhibit self confidence has the ability to trust his or her own personal judgement.

Integrity

A concept that has to do with a personal feeling of "wholeness".

The Patient Bill of Rights in Medicare and Medicaid.

Medicare now requires that patients be informed of their rights, including the right to know what treatment, and the right to confidentiality.

Confidentiality is such an important issue in testing for HIV that the patient must sign a ____

Consent form before the specimen for the test can be collected.

The Health Insurance Portability and Accountability Act (HIPPA) of 1996.

A federal law was passed requiring all healthcare providers to obtain as patients consent in writing before disclosing medical information.

Communications defined

The means by which information is exchanged or transmitted.

Kinesics

The study of nonverbal communication. It includes characteristics of body motion and language such as facial expression, gestures, and eye contact.

Kinesic slip

When verbal and nonverbal messages do not match.

Proxemics

The study of an individual's concept and use of space.

Two general categories of facilities.

Inpatient (Nonambulatory) and Outpatient (Ambulatory)

Three levels of healthcare

Primary, Secondary, and Tertiary

Public Health Service (PHS)

To promote the protection and advancement of the nation's physical and mental health.

A third party payer.

Can be an insurance company, the federal government, a managed care program, or a self-insured company that pays for healthcare services on behalf of its employees.

Today's large managed care organizations (MCOs) evolved from prepaid healthcare plans such as ______

health maintenance organizations (HMOs) and preferred provider organizations (PPOs)

Integrated healthcare delivery systems (IDSs)

A healthcare provider made up of a number of associated medical facilities that furnish coordinated healthcare services from prebirth to death.

Hematology

The hematology department performs laboratory tests that identify diseases associated with blood and blood-forming tissues.

Coagulation

Coagulation is the study of the ability of blood to form and dissolve clots.

Chemistry

The chemistry department performs most laboratory tests.

Serology or Immunology

The term serology means the study of serum. Serology tests deal with the body's response to the presence of bacterial, viral, fungal, or parasitic diseases stimulating antigen-antibody reactions that can easily be demonstrated in the laboratory.

Urinalysis (UA)

maybe housed in hematology or chemistry area.

Microbiology

The microbiology department analyzes body fluids and tissues for the presence of microorganisms, primarily by means of culture and sensitivity (C&S) testing.

Blood Bank (Immunohematology)

The blood bank or immunohematology department of the laboratory prepares blood products to be used for patient transfusions.

Histology

Defined as the study of the microscopic structure of tissues.

Cytology

Cytology tests are concerned with the structure of cells.

Cygogenetics

In this section, samples are examined for chromosomal deficiences that relate to genetic disease.

Specimens used for chromosomal studies include:

Tissue, blood, and amniotic fluid.

Reference Laboratories

Large independent laboratories that receive specimens from many different facilities located in the same city, other cities in the same state, or even cities that are out of state.

Laboratory Director/Pathologist

The pathologist is a physician who specializes in diagnosing disease, through the use of laboratory tests results, in tissues removed at operations from postmortem examinations.

Laboratory Administrator/Laboratory Manager

The lab administrator is usually the technologist with an advanced degree and several years of experience.

Duties of the administrator include:

overseeing all operations involving physician and patient services.

Technical Supervisor

Is responsible for the administration of the area and who reports to the laboratory administrator.

Medical Technologist/Medical Laboratory Scientist

Generally has a bachelor's (BS) degree plus additional studies and experience in clinical laboratory testing.

The Clinical Laboratory Improvement Amendments of 1988 (CLIA '88)

Is a federal law that allows the Centers for Medicare and Medicaid services to regulate all sites performing laboratory testing in the United States.

Hematocrit

Abbreviation: HctTube: LavenderValues correspond to the red cell count and hemaglobin level; when decreased indicates anemic conditions.

Hemoglobin

Abbreviation: HgbTube: LavenderDecreased values indicate anemic conditions; values normally differ with age, sex, altitude, and hydration.

Red blood cell count

Abbreviation: RBC countTube: LavenderMeasure of erythropoietic activity; decreases in numbers are related to anemic conditions.

White blood cell count

Abbreviation: WBC countTube: LavenderAbnormal leukocyte response indicative of various conditions, such as infections and malignancies; When accompanied by low WBCs, differential test becomes more specific.

Platelet Count

Abbreviation: Plt Ct.Tube: LavenderDecreased numbers are indicative of hemorrhagic diseases; values may be used to monitor chemotherapy or radiation treatments.

Differential white count

Abbreviations: DiffTube: LavenderChanges in the appearance or number of specific cell types signify specific disease conditions; values are also used to monitor chemotherapy or radiation treatments.

Indices

Changes in RBC size, weight, and Hgb content indicate certain types of anemias.

Mean corpuscular hemoglobin

Abbreviation: MCHTube: LavenderReveals the weight of the hemoglobin in the cell, regardless of size.

mean corpuscular volume

Abbreviation: MCVTube: LavenderReveals the size of the cell, Decreased MCV is associated with thalassemia and iron-deficiency anemia.

Mean corpuscular hemoglobin concentration

Abbreviation: MCHCTube: LavenderReveals the hemoglobin concentration per unit volume of RBCs.

Red blood cell distribution width

Abbreviation: RDWTube: LavenderReveals the size differences of the RBCs.

Bone marrow

Tube: LavenderDetects abnormal blood cells and evaluates blood cell formation and function.

Cerebrospinal and other body fluids

Abbreviation: CSFTube: LavenderPresence or absence, number and type of cells. Hematocrit on fluid indirectly measures fluid volume.

Eosinophil count

Tube: LavenderIncreased numbers in direct count indicate parasitic infections and allergies.

Erythrocyte sedimentation rate

Abbreviation: ESRTube: LavenderIncreased rate at which red blood cells settle out is indicative of inflammatory conditions or necrosis of tissue.

Lupus erythematosus

Abbreviation: LE cellsTube: Lavenderpresence of typical LE cells is diagnostic of systemic LE.

Osmotic fragility

Tube: LavenderIncreased red cell fragility is indicative of hemolytic and autoimmune anemias.

Reticulocyte (retic) count

Abbreviation: retic countTube: LavenderIncreased number of retics in circulating blood attest to bone marrow hyperactivity.

Sick cell screen

Tube: LavenderSickling of red cells indicates presence of Hgb S, and abnormal hemoglobin variant.

amino acid

Building blocks of protein

anemia

A condition in which the blood is deficient in red blood cells, in hemoglobin, or in total volume.

antecubital space

space located at the bend in the elbow

anticoagulant

a drug that prevents clotting of the blood

antigen

A protein that, when introduced in the blood, triggers the production of an antibody

arterial blood

They take blood from an artery. Done by respiratory. It is an arterial blood specimen done at the hand or forearm. Looks at - Oxygen carried in the blood, Carbon dioxide carried in the blood, pH level. Following an ABG make sure that there is no bleeding in the site afterwards.

aseptic technique

-Includes all activities to prevent or break the chain of infection

biohazardous

items contaminated with blood, body fluids, secretions, or excretions

blood culture tube

This test is collected in a tube with a light-blue topthe first order of draw recommended by the NCCLS (2004)

blood type test

determine blood type and compatibility

CLIA waived test

Tests that provide simple, unvarying results and require minimal amount of judgment and interpretation

CPR

CARDIOPULMONARY RESUSCITATION

capillary

A tiny blood vessel where substances are exchanged between the blood and the body cells.

centrifuge

A device that spins test tubes of blood

chemical additive

can prevent growth of microbes, but may be toxic

cholesterol

A type of fat made by the body from saturated fat; a minor part of fat in foods.<200 mg/d

coagulation tube

has a blue stopper, and is used when drawing blood for coagulation (clotting) studies, such as prothrombin time (PT)

complete blood count

The number of RBC's (red blood cells), WBC's (white blood cells) and platelets (per cubic millimeter) that are present in the patient's sample of blood is determined.

congenital disease

disease that exists at birth.

contaminate

To harm by contact with something undesirable

disinfection

A process that eliminates many or all microorganisms, with the exception of bacteria spores, from inanimate objects

EDTA tube

for hemoglobin A1c test

Erythrocyte Sedimentation Rate

ESR; TEST THAT MEASURES THE SPEED AT WHICH RBC'S SETTLE OUT IN ANTICOAGULATED BLOOD

fasting

Nothing to eat or drink for at least 12 hours.

fasting blood sugar test

measures the glucose (blood sugar) levels after the patient has not eaten for 8-12 hours

glucose

70-110

glucose tolerance test

Fasting = 60-110 mm/dL, 1 hour = 190 mm/dL, 2 hours = 140 mm/dL, 3 hours = 125 mm/dL

glycohemoglobin test

determine the control of pt blood sugar in the last 2-3 months

HIV

A virus that attacks and destroys the human immune system.

hematocrit

% of total blood volume that is cells

hematoma

A localized swelling filled with blood

hemoglobin

(f) 12-16 ; (m) 14 - 18A four-subunit protein found in red blood cells that binds oxygen. Each subunit contains a heme group, a large multi-ring molecule with an iron atom at its center. One hemoglobin molecule can bind four oxygen molecules in a cooperative manner.

hemolysis

Destruction of blood (breakdown of red blood cells with release of hemoglobin).

hepatitis B

A form of hepatitis that is transmitted through contact with infected blood, body fluids (sexually transmitted), contaminated needles

insulin

A hormone produced by the pancreas or taken as a medication by many diabetics

lancet

SMALL, SHARP INSTRUMENT USED TO PERFORM CAPILLARY PUNCTURES

metabolism

All of the chemical reactions that occur within an organism.

microorganism

A small living plant or animal seen only with a microscope; a microbe

nonadditive tube

name the tube that does not need mixing

order of draw

1. Yellow, 2. Light Blue, 3. Red, 4. Gold, Tiger Top, Red/Yellow/Black, 5. Green, Light Green, Green/Gray, 6. Purple/Lavender, 7. Pink, 8. Gray, 9. Royal Blue, 10. Tan

phenylketonuria

A rare inborn error of phenylalanine metabolism. Inherited as an autosomal recessive trait. Accumulation of phenylalanine in untreated cases causes CNS toxicity, most notably mental deficiency; organ damage; unusual posture; and can, in cases of maternal, severely compromise pregnancy PKU

phlebotomy

Incision of a vein

plasma

1. Yellow liquid component of blood in which the blood cells in whole blood are normally suspended 2. It makes up about 55% of the total blood volume 3. Intravascular fluid part of extracellular fluid 4. mostly water (93% by volume) and contains dissolved proteins, glucose, clotting factors, mineral ions, hormones and carbon dioxide

plasma separating tube

Light Green Tube Top (PST) additive

platelets

150,000-400,000Blood clotting

polycythemia

A disorder characterized by an abnormal increase in the number of red blood cells in the blood

separator gel

Used to create a physical barrier between the serum and the cells; after collection, tube is inverted 5 times, blood allowed to clot for 30 minutes, then centrifuged; during centrifugation, the gel positions between the serum and cell layers. The gel also contains a clot activator

specimen

a bit of tissue or blood or urine that is taken for diagnostic purposes

standard precautions

A strict form of infection control that is based on the assumption that all blood and other body fluids are infectious.

tourniquet

A device used for bleeding control that constricts all blood flow to and from an extremity.

urinate

excrete or expel urine

vaccine

A harmless variant or derivative of a pathogen that stimulates a host's immune system to mount defenses against the pathogen

venipuncture

Entering the skin with a needle, generally to draw a blood sample.

venous blood

BLOOD OBTAINED FROM A VEIN; OXYGEN-DEPLETED BLOOD WHICH IS DARKER THAN ARTERIAL BLOOD.

The value of leach therapy lies in the components of the worm's_____.

Saliva; which contains a local vasodilator, a local anethetic, and hirudin (an anticoagulant)

Polycythemia

a disorder involving the overproduction of red blood cells.

Certification

A voluntary process by which an agency grants recognition to an individual who has met certain prerequisites in a particular technical area.

Licensure

an official document or permit granted by a state agency that gives legal permission for a person to work in a particular health profession.

Continuing Education

Designed to update the knowledge or skills of participants and is generally geared to a learning activity or course of study for a specific group of health professionals, such as phlebotomists.

By recognizing diversity

The phlebotomist promotes goodwill and harmonious relationships that directly improve health outcomes, the quality of services, and public relations.

Professionalism

Is defined as the conduct and qualities that characterize a professional person.

Self confidence

Phlebotomists who exhibit self confidence has the ability to trust his or her own personal judgement.

Integrity

A concept that has to do with a personal feeling of "wholeness".

The Patient Bill of Rights in Medicare and Medicaid.

Medicare now requires that patients be informed of their rights, including the right to know what treatment, and the right to confidentiality.

Confidentiality is such an important issue in testing for HIV that the patient must sign a ____

Consent form before the specimen for the test can be collected.

The Health Insurance Portability and Accountability Act (HIPPA) of 1996.

A federal law was passed requiring all healthcare providers to obtain as patients consent in writing before disclosing medical information.

Communications defined

The means by which information is exchanged or transmitted.

Kinesics

The study of nonverbal communication. It includes characteristics of body motion and language such as facial expression, gestures, and eye contact.

Kinesic slip

When verbal and nonverbal messages do not match.

Proxemics

The study of an individual's concept and use of space.

Two general categories of facilities.

Inpatient (Nonambulatory) and Outpatient (Ambulatory)

Three levels of healthcare

Primary, Secondary, and Tertiary

Public Health Service (PHS)

To promote the protection and advancement of the nation's physical and mental health.

A third party payer.

Can be an insurance company, the federal government, a managed care program, or a self-insured company that pays for healthcare services on behalf of its employees.

Today's large managed care organizations (MCOs) evolved from prepaid healthcare plans such as ______

health maintenance organizations (HMOs) and preferred provider organizations (PPOs)

Integrated healthcare delivery systems (IDSs)

A healthcare provider made up of a number of associated medical facilities that furnish coordinated healthcare services from prebirth to death.

Hematology

The hematology department performs laboratory tests that identify diseases associated with blood and blood-forming tissues.

Coagulation

Coagulation is the study of the ability of blood to form and dissolve clots.

Chemistry

The chemistry department performs most laboratory tests.

Serology or Immunology

The term serology means the study of serum. Serology tests deal with the body's response to the presence of bacterial, viral, fungal, or parasitic diseases stimulating antigen-antibody reactions that can easily be demonstrated in the laboratory.

Urinalysis (UA)

maybe housed in hematology or chemistry area.

Microbiology

The microbiology department analyzes body fluids and tissues for the presence of microorganisms, primarily by means of culture and sensitivity (C&S) testing.

Blood Bank (Immunohematology)

The blood bank or immunohematology department of the laboratory prepares blood products to be used for patient transfusions.

Histology

Defined as the study of the microscopic structure of tissues.

Cytology

Cytology tests are concerned with the structure of cells.

Cygogenetics

In this section, samples are examined for chromosomal deficiences that relate to genetic disease.

Specimens used for chromosomal studies include:

Tissue, blood, and amniotic fluid.

Reference Laboratories

Large independent laboratories that receive specimens from many different facilities located in the same city, other cities in the same state, or even cities that are out of state.

Laboratory Director/Pathologist

The pathologist is a physician who specializes in diagnosing disease, through the use of laboratory tests results, in tissues removed at operations from postmortem examinations.

Laboratory Administrator/Laboratory Manager

The lab administrator is usually the technologist with an advanced degree and several years of experience.

Duties of the administrator include:

overseeing all operations involving physician and patient services.

Technical Supervisor

Is responsible for the administration of the area and who reports to the laboratory administrator.

Medical Technologist/Medical Laboratory Scientist

Generally has a bachelor's (BS) degree plus additional studies and experience in clinical laboratory testing.

The Clinical Laboratory Improvement Amendments of 1988 (CLIA '88)

Is a federal law that allows the Centers for Medicare and Medicaid services to regulate all sites performing laboratory testing in the United States.

EPA

Environmental Protection Agency federal that regulates the disposal of hazardous waste.

RACE

RescueAlarmConfineExtinguish

Hemochromatosis

To much iron

Engineering controls

Devices that isolate or remove a blood borne pathogen hazard from the workplace.

Chain of infection

Number of events in a series, lead to an infection.

CDC

Centers for Disease Control Prevention, division of the U.S. Public Health Service They investigate and control disease w epidemic potential.

Boihazard

Anything potentially harmful to health.

BBP

Blood borne pathogen

IDN

Integrated delivery networks

Polycythemia

Disorder of overproduction of RBC's

TherapeuticReasons

Removal of bloodPolycythemia, Hemochromatosis

PM

Personalized medicine

PCP

Primary Care Physician

Kinesics

Study of nonverbal communication

Kinetic slip

When verbal and nonverbal do not match

Medicaid

Federal and State program that provides medical assistance for low-income Americans

Medicare

Federally funded program that provides healthcare to people over age 65 and disabled

Proxemics

Study of an individual's concept and use of space

MCO

Managed Care Organization

PHI

Protected health information

MLS

Medical Lab Scientist

PHS

Public Health Service

Primary care

Care by General physician who assumes on going responsibility for maintaining a patient's health

Secondary care

Care by a physician (specialist) who can perform out-of-the-ordinary procedures in outpatient facilities

Reference Laboratories

Large independent laboratories that receive and test specimens from many different facilities

Phlebotomy

Incision into the vein for the purpose of drawing blood or Venesection

Tertiary Care

Specialized health care for inpatients

Standard of Care

Normal level of skill and care a healthcare practitioner would provide for their patients

Respondent Superior

Latin ,``let the master respond``. Employers must answer for damages their employees cause

Quality indicators

Guides used as monitors of all areas of patient care

QC

Quality control Specific activities/techniques to fulfill requirements for a quality service/product.

QA

Quality assurance Processes used to create standard quality service/prevention of problems.

Plaintiff

Injured party in the litigation process

PSC

Patient Service Centers

NPSG

National Patient Safety Goals

Negligence

Failure to exercise due care

Malpractice

Type of negligence committed by a professional

Invasion of Privacy

Violation of one's right to be left alone

Implies voluntary and competent permission for a medical procedure, test or meds

IQCP

Individualized Quality Control Plan

Due care

The level of care a person w intelligence and good sense would exercise under the circumstances

GLP

Good Laboratory Practices

Discovery

Formal process in litigation that involves taking depositions and interrogating the parties involved

Fraud

Deceitful practice or false portrayal of facts by either words or conduct

Deposition

Process in which one party questions another under oath while a court reporter records every word

Delta check

Comparison of current results of a lab test w previous results for the same test and patient

Defendant

In a lawsuit, a person against whom a complaint is filed

CQI

Continuous Quality Improvement

CLSI

Clinical and Laboratory Standards Institute

Civil actions

Legal actions in which the alleged injured party sues for monetary damages

Breach of confidentiality

Failure to keep privileged medical information private

Battery

Intentional harmful or offensive touching or use of force on a person without consent or legal justification

Assault

Act or threat causing another to be in fear of immediate battery

Statue of Limitations

Law setting the length of time an alleged injury which the injured person is permitted to file a lawsuit

TJC

The Joint CommissionOrganization, establishing standards for hospitals/health related facilities.

Threshold Values

Level of acceptable practice beyond which quality patient care cannot be assured

Tort

Wrongful act committed against one's person, property, reputation, or legally protected right

ACA

Affordable Care Act

ACO

Accountable Care Organization

CLIA ''88''

Clinical Laboratory Improvement Amendments of 1988

AHCCCS

Arizona Health Care Cost Containment System

Certification

Evidence an individual has Mastered fundamental competencies in a particular technical area

Vicarious liability

Liability imposed by law on one person for acts committed by another

HMO

Health Maintenance Organization

CMS

Center for Medicare and Medicaid Services

CPT

Current Procedural Terminology

Exsanguinate

To remove all blood

Case Manager

person who coordinates medical services on behalf of a patient

HIPAA

Health Insurance Portability and Accountability Act

Communication barriers

Biases or personalized filters that are major obstructions to verbal communication

ICD-10-PCS

International Classification of Diseases, Revision Procedural Coding System

Isolation procedures

Procedures to separate patients with transmissible infections from contact with others.

Standard precautions

precautions intended to minimize risk of infection transmission when caring for all patients.

Susceptible host

Individual who has little resistance to an infectious agent.

Transmission based precautions

precautions used with standard precautions for patients known or suspected to be infected with highly transmissible significant pathogens

Infectious / causative agent

pathogen responsible for causing an infection. Also called causative agent

Immune

Protected or resistant to a particular disease/infection because of development of antibody's through vaccination or recovery from the disease.

HIV

Human Immunodeficiency Virus

HICPAC

Healthcare Infection Control Practices Advisory Committee

HCV

Hepatitis C Virus

HCS

Hazard Communication Standard

HBV

Hepatitis B Virus

HAI

Healthcare Associated Infection

Fomites

Countertops and computer keyboards that can harbor infectious agents.

PPE

Personal Protection Equipment

Pictogram

Universally accepted symbolsrepresenting a word or idea by illustration.

Permucosal

Through mucous membranes

Neutropenic

Abnormal small number of neutrophils in the blood

NHSN

National Healthcare Safety Network

NIOSH

National Institute for Occupational Safety and Health

Nosocomial infection

An infection acquired in a healthcare facility

OSHA

Occupational Safety and Health AdministrationU.S. gov't agency that mandates and enforces safe working conditions for employees.

Parenteral

Administration by any route other than digestive tract. Example: intravenous or intramuscular injection

Pathogens/Pathogenic

Microbes capable of causing disease

Percutaneous

Through the skin

Vehicle transmission

infectious disease spread through contaminated food, water, drugs, or blood transfusion.

Work practice controls

Practices used to reduce the likelihood of bloodborne pathogen exposure

Vector transmission

infectious disease spread by insect, anthropod (exoskeleton), or animal.

Microbe

short for microorganismNot visible to the naked eye.

Asepsis

Absence of bacteria, viruses, and other microorganisms, typically during surgery.

Fire Tetrahedron

Triangle of fire components Heat, Fuel, Oxygen, Chemical

Reservoir

source of an infectious microorganism

SDS

Safety Data Sheets

Reverse isolation

Protective isolation for patients with compromised health conditions. Example: Burn victims, HIV, etc.

Class AClass BClass CClass DClass K

OrdinaryFlammableElectricalCombustible metalsKitchen, oils

Light blue

Sodium citrateCoagulation DeptPT, aPTT, bleeding time, dDimer(frozen),Fibrinogen(frozen)INR- international normalized ratio(TT)-thrombin time(FSP)Fibrin split products

Red (glass)

No additiveChemistry, Blood BankSerology/Immunology

Red (plastic)

Clot activator/ gel barrierChemistryBilirubin(fridge,foil),Creatinine(kidney,fridge)

Red Tiger Top

Clot activator & Gel separatorChemistryBUN/creatine/glucose

Green/grayLight green

Lithium heparin & Gel separatorChemistry

Green

Lithium heparinSodium heparinChemistrySTAT Plasma separator

Lavender

EDTAHematology WBC,RBC,Hgb,Hct, Platelet,diff ct., Eryth. sed.rate,MCV, MCH, MCHC globin, RDW-red dist. WidthAmmonia(Ice,chemistry)

Pink

EDTABlood Bank/Immunohemotology

Gray

Sodium fluoride & potassium oxalateSodium fluoride & EDTASodium fluorideChemistryGlucose

Royal blue

NoneEDTASodium heparinChemistry

Tan

EDTAChemistryleads

Yellow

Sodium polyanethol sulfonate (SPS)Microbiology

Yellow

Acid citrate dextrose (ACD)Blood Bank/Immunohematology

Anticoagulant

Substance that prevents blood from clotting

Antiglycolytic agent

Prevents glycolysis (glucose breakdown)

Additive

Substance within a tube

ACD

Acid citrate dextrose (anticoagulant)

Hemostasis

After injury coagulation process

Fibrinolysis

Stage 4 homeostasisRemoval of blood clot once healing starts

Erythrocyte

RBCCarry oxygen from lungs to cells Transport CO2 away from cell to lungs

Systemic circulation

Pathway carries oxygenated blood from heartReturns blood to heart carrying CO2

Serum

Clear pale fluidCan be separated from blood specimenNo fibrinogen

Pulmonary circulation

Pathway that carries CO2 blood from heart to lungsWhere CO2 is removed, returns oxygenated blood to the heart

Plasma

Clear fluid Contains fibrinogen

Median cubical vein

1st choiceMiddle location ofantecubital fossa

Leukocyte

WBC nucleus cell function to fight infection

Extrinsic

Coagulation pathway starts by release of thromboplastinfrom injury

Intrinsic

Coagulation factors circulating the bloodstream

Atria

Upper receiving chambers on each side of heart

Arrhythmia

Irregular heart rhythm

Antecubital veins

Major superficial veins in antecubital fossa

Blood pressure

Measure of force exerted by the blood on walls of blood vessels

Basilic vein

Last choice, large vein on inner side of antecubital area

Cardiac cycle

One complete contraction & relaxation of the heart

Cephalic vein

2nd choice, located lateral of antecubital fossa

Coagulation

Blood clotting process

Cross match

Test to determine compatibility of donor & recipient blood or tissue

Diastole

relaxing phase of cardiac cycle

Systole

contracting phase of cardiac cycle

Thrombin

enzyme that converts fibrinogen into fibrin necessary for clot formation

Thrombocyte

Med term for platelets, help coagulate, smallest of formed elements

Vasoconstriction

Stage 1 of hemostasis, damaged vessel narrows to decrease flow of blood to injury

Ventricles

lower pumping, delivering chambers on each side of heart

Winged infusion set

1/2 - 3/4 inch stainless steel needle connected to 5-12 inch tube w multisample luer adapter for evacuated tubes (Butterfly Needle) 23 gauge common

Thixotropic gel

non-reacting synthetic gel in ETS tubes, forms barrier between cells & serum or plasma when centrifuged

SST

serum separator tubes

SPS

sodium polyanethol sulfonate, anticoagulant, reduces action of protein called complement, which destroys bacteria

Sodium fluoride

preserves glucose & inhibits growth of bacteria

Sodium citrate

anticoagulant, prevents clotting by binding calcium, preserves coagulation factors

Silica

glass particles used to enhance coagulation

Sharps container

special container to dispose sharps, needles

Shaft

long cylinder portion of needle

PST

plasma separator tube

Potassium oxalate

anticoagulant commonly used w antiglycolytic agent sodium fluoride

Order of draw

special sequence, minimize additive cross-contamination

Multisample needle

needle that allows multiple tubes to be collected

Lumen

inner space of a blood vessel or tube

Hypodermic needle

needle used with syringe

Hub

threaded end where needle attaches

Heparin

anitcoagulant prevents clotting, inhibits thrombin formation

Glycolysis

breakdown of glucose by blood cells

Gauge

diameter of lumen

Evacuated tube

premeasured vacuum, color coded w additive inside

ETS

evacuated tube system, blood flows directly from vein to tube

EDTA

ethylenediaminetetraaacetic acid, anticoagulant, hematology, preserves cells & platelet clumping

Disinfectant

solutions used to kill microorganisms

clot activator

enhances coagulation

Bevel

point of needle, cut on slant for skin entry

Antiseptic

inhibits growth of bacteria, cleans skin

Stat

immediately

Requisition

form test orders are entered & sent to lab

reflux

backflow, blood into vein from collection tube during venipuncture

preop/postop

before surgery / after surgery

patient ID

process verifying patient's ID

patency

freely open, normal condition of a vein

palpate

examine by feel or touch

NPO

Nothing by mouth

needle sheath

needle cover

needle phobia

intense fear of needles

MR number

Medical record number for patient

ID card

clinic issued ID document

ID Band / bracelet

Identification

hospice

care of terminally ill

fasting

No food / drink for 12 hrs

EMLA

easily melted mix of local anesthetics

DNR / DNAR

Do Not Resuscitate / Do Not Attempt Resuscitate

bedside manner

behavior of healthcare provider toward patient

bar code

series black stripes, spaces, corresponds to letters & numbers

ASAP

as soon as possible

arm / wrist band

Two other names for ID band / bracelet

anchor

secure firmly

accession

process, recording in order received

Hemostatic Process

VasoconstrictionPrimary platelet plug formationSecondary hemostatic plug formationFibrinolysis

A-line

catheter placed in artery for(blood pressure, collect gas, blood specimens, administer drugs)

AV shunt fistula graft

permanent artery & vein fusion, access for dialysis. permanent artery & vein fusion,create access for dialysis.surgical connection of artery & vein using vein or tubing

bariatric

treatment of obesity

basal state

resting metabolic state, morning after fasting, 12 hrs

bilirubin

breakdown of RBC's

CVAD

central vascular access device

CVC

central venous catheter

diurnal

happening daily

circadian

biologic rhythms, having 24hr cycle

edema

swelling, abnormal fluid in tissues

exsanguination

blood loss, life cannot be sustained

hematoma

clotted blood following venipuncture

hemoconcentration

decrease of fluid content in blood(red blood cells)

hemolysis

damage of RBC's, (pink color, slight hemolysis, red, gross hemolysis)

iatrogenic

adjective used to describe condition by effects of treatment

icteric

"marked by jaundice", abnormal yellow color from bilirubin levels

implanted port

chamber, permanent, indwelling line under skin, upper chest or arm

IV

within a vein

jaundice

also called icterus; increased bilirubin, breakdown RBC's.

lipemic

serum/plasma, milky, due to high lipid content

lymphostasis

obstruction of normal lymph flow

petechiae

tiny red spots with tourniquet, capillary wall defect or platelet abnormalities

PICC

peripherally inserted central catheter

preanalytical

prior to analysis

reference ranges

normal lab values for healthy individuals

saline lock

catheter in vein( access to meds, drawing blood for 48 hrs)

sclerosed

hardened

syncope

medical term for fainting

thrombosed

vessel that is clotted

vasovagal

sudden faintness from nervous system(pain, stress, trauma)

venous stasis

trapping blood by vein compression

BMP

Basic metabolic panelGlucose, blood urea nitrogen (BUN), creatinine, sodium, potassium, chloride, CO2, calcium

CMP

Comprehensive metabolic panelAlbumin, glucose, BUN, creatinine, sodium, potassium, chloride, CO2, AST, ALT, total protein, total bilirubin, calcium

Electrolyte Panel

sodium, potassium, chloride, CO2

Hepatic function panel A

AST, ALT, alkaline phosphatase, total protein, albumin, total bilirubin, direct bilirubin

Lipid panel

Cholesterol, lipoprotein, high-density cholesterol(HDL)Triglycerides

Renal function panel

Glucose, BUN, creatinine, sodium, potassium, chloride, CO2, calcium, albumin, phosphorus

"Arterialized" means

Arterial content has been increased

A blood smear is:

Blood spread out on a microscope slide

A cyanotic extremity would:

Be bluish in color

After making a blood smear:

Let it dry naturally

An acceptable routine blood smear:

Has a feathered edge

Capillary specimens contain:

All of the above: Arterial blood, tissue fluids, venous blood

CBG specimens are collected in:

Narrow-bore capillary tubes

Correct newborn screening test collection of handling includeds:

Using one large drop to entirely fill a collection circle

Errors in capillary glucose results have been attributed to:

Isopropanol contamination of the specimen

Falsely decreased bilirubin results can be caused by:

Failing to protect the specimen from light

It is inappropriate to apply a bandage to a capillary puncture site on an infant or child younger than 2 years of age because it can:

All of the above: Irritate the infant's tender skin, pull off & be a chocking hazard, tear delicate skin when removed

Neonatal screening is the testing of:

Newborns for certain disorders

Osteochondritis is:

Inflammation of the bone and cartilage

Phenylketonuria (PKU) is a:

Genetic disorder involving phenylalanine metabolism

Proper finger puncture technique would exclude:

Puncturing parallel to the fingerprint

Test affected by povidone-iodine contamination of a capillary specimen include:

All of the above; Phosphorus, Potassium, Uric Acid

The major blood vessels of the skin are located:

At the dermal-subcutaneous junction

Which of the following are required characteristics of capillary puncture lancets?

All of the above: A controlled depth of puncture, Blades or points that are sterile, Permanently retractable blades

Which of the following capillary puncture techniques is incorrect?

Press the lancet down into the skin so it does not slip

Which of the following is least likely to contaminate a PKU test?

Using isopropyl alcohol to clean the site

Which statement concerning capillary blood gases is untrue?

Results are much more accurate than ABGs

Spiral patterns of fingerprints

arterialized

capillary blood increased by warming site

blood film/smear

blood drop spread thinly on slide

calcaneus

heel bone

CBG's

capillary blood gases

cyanotic

blue color from lack of oxygen

differential

under microscope test to determine number, type & characteristics of blood cells

feather

thinnest area of blood smear where differential is performed

galactosemia

disorder caused by lack of enzyme needed to convert milk sugar galactose into glucose

hypothyroidism

disorder, having insufficient thyroid hormones

interstitial fluid

fluid in tissue spaces between cells

intracellular fluid

fluid within the cells

lancet

sterile, disposable, sharp-pointed instrument that punctures skin for capillary blood specimens

microcollection containers or Microtubes

small plastic tubes to collect blood obtained from capillary puncture Bullets

microhematocrit tubes

disposable, narrow, capillary tubes that fill by capillary action

neonatal screening

routine test for newborns presence of metabolic & genetic disorder example: phenylketonuria

osteochondritis

inflammation of the bone & cartilage

osteomyelitis

inflammation of bone marrow & adjacent bone

PKU

Phenylketonuria defect in metabolism of phenylalanine

planter surface

bottom of foot

posterior curvature

back of heel

whorls

spiral pattern of ridges & grooves that form a fingerprint

AABB

American Association of Blood Bank

ACT

Activated clotting time

aerobic

with air

anaerobic

without air

ARD

Antimicrobial removal device

autologous

Blood that is donated for one's own use

BAC

Blood alcohol concentration

bacteremia

bacteria in the blood

BNP

B-type natriuretic peptide

Chain of custody

special strict protocol for forensic specimens that requires detailed documentation tracking the specimen from the time it's collected until results are reported

Compatibility

ability to be mixed together; blood transfusions

CRP

C-reactive protein

EQC

electronic quality control

ETOH

Abbreviation for ethanol or blood alcohol

FAN

Fastidious antimicrobial neutralization

FUO

Fever of unknown origin

GTT

Glucose tolerance test

hCG

Human chorionic gonadotropin

hypoglycemia

condition where the level of glucose sugar in the blood is low

hypokalemia

low potassium in the blood

hyponatremia

low sodium in the blood

hypoxemia

low level of oxygen in the blood

iCa2+

Ionized calcium

INR

International normalized ratio

K+

Potassium

lactate

A form of lactic acid that is used as a marker for severity of metabolic acidosis and stress response

lookback

Program that requires all components of a unit of blood to be traceable back to the donor and requires notification to blood recipients who received, turned positive, for a transmissible disease

Lysis

rupturing/bursting of RBC's

NIDA

National Institute on Drug Abuse

peak level

Drug level collected when the highest serum concentration of the drug is anticipated

POCT

Point of Care Testing

PP

Postprandial

septicemia

Microorganisms/toxins in the blood

TDM

Therapeutic drug monitoring

TGC

Tight glycemic index

Tnl

Troponin I, protein specific to heart muscle used in diagnosing an acute myocardial infarction/heart attack

TnT

Troponin T, protein specific to heart muscle, heart attack and to monitor effectiveness of thrombolytic therapy

trough level

drug level collected when the lowest serum concentration of the drug is expected, usually prior to the next scheduled dose.

AFP

Alpha-fetoprotein

Amniotic fluid

Clear fluid fills the membrane surrounding/ cushioning fetus in uterus

buccal swab

swab material collected from the inside of the cheek

C&S

culture & sensitivity

C. difficile

bacterium that causes symptoms from diarrhea to life-threatening inflammation of the colon

catheterized

urine specimen collected from sterile catheter inserted through urethra into bladder

clean-catch

method of obtaining a urine sample that is free of contamination

CSF

Cerebrospinal fluid

expectorate

spit; act of ejecting saliva from mouth

FIT

Fecal immunochemical test/ fecal occult blood test

FOBT

fecal occult blood test

gastric analysis

test that examines stomach contents abnormalities & measures gastric acid concentration to evaluate stomach acid production

H. pylori

Helicobacter pylori, Bacterial secreting substances that damage lining of stomach & cause chronic gastritis which can lead to peptic ulcer disease

iontophoresis

electrical stimulation from electrodes placed on skin for production of sweat in the sweat chloride test

midstream

urine collection in middle of urination rather than beginning or end

NP

Nasopharyngeal

O&P

Ova & parasites test to detect intestinal parasites & eggs in feces

occult blood

Hidden blood in feces

PCR

Polymerase chain reaction

pericardial fluid

fluid aspirated from the pericardial cavity that surrounds the heart

peritoneal fluid

fluid aspirated from the abdominal cavity

pleural fluid

fluid aspirated from the pleural cavity surrounding the lungs

serous fluid

pale-yellow watery fluid found between double layered membranes that enclose the pleural, pericardial, peritoneal cavities

sputum

mucus or phlegm ejected from trachea, bronchi, lungs by deep coughing

suprapubic

obtaining uncontaminated bladder urine directly from inserting needle directly into bladder

sweat chloride

test of sweat production stimulation by electrical means then measuring chloride content to diagnose cystic fibrosis

synovial fluid

viscid (sticky) colorless fluid found in joint cavities

24-hour urine

pooled urine collected over a 24 hr period, beginning in the morning

UA

Urinalysis

UTI

Urinary tract infection

abducted

away from the body; position of arm for arterial blood gas collection

ABG

arterial blood gases

Allen test

noninvasive test to assess collateral circulation before collecting blood specimen from radial artery

arteriospasm

reflex involuntary contraction of the artery that can be caused by pain or irritation during needle penetration of artery muscle or result of anxiety during arterial puncture

brachial artery

Artery located in the medial anterior aspect of the antecubital fossa near insertion of the bicep muscle; 2nd choice for arterial puncture

collateral circulation

area supplied with blood from more than one artery so circulation can be maintained if one vessel is obstructed

femoral artery

large artery located in the groin, lateral to the pubic bone, used for arterial puncture

FiO2

fraction of inspired oxygen, as in oxygen therapy

L/M

liters per minute, in oxygen therapy

radial artery

located at the thumb side of the wrist, 1st choice, common site for arterial puncture

steady state

stable condition required in order to obtain blood gas specimens, condition where no exercise, suctioning, respirator change for 20-30 minutes

ulnar artery

located on medial aspect / little finger side of the wrist

Accession number

Number generated by laboratory information system (LIS) when specimen request is entered into the computer

Aerosol

Fine mist of specimen

Aliquot

Portion of specimen used for testing

Bar code

Series of black stripes & white spaces of varying widths that correspond to letters and numbers

Biobank

Bank where human biological samples, blood, saliva, plasma, skin cells, organ tissues can be stored & used in research

Breach

Failure to keep medical information private

Central processing

Screening & prioritizing area where specimens are received & prepared for testing

Centrifuge

Machine that spins blood tubes at a high number of revolutions per minute

Cloud

permanent secondary storage

Cursor

flashing indicator

Data

Information collected for analysis

DOT

Department of transportation

EMR

electronic medical records

Hardware

Computer equipment used to process data

HIS

hospital information system

IATA

International Air Transport Association

Icon

Images used to request appropriate programs or functions on computer

ID code

Unique identification for users

Input

Enter data into computer

Interface

Connect for the purpose of interaction

LIS

Laboratory information system

Measurand

quality/amount of substance measured

list of options

Middleware

software "glued together" two separate, already existing

Mnemonic

memory aiding code or abbreviation

Network

group of computers linked for sharing resources

Output

return of processed information or data to user or another location

Password

code that identifies a person and allows them to be a user

Postcentrifugation

after centrifuge

Preanalytical

prior to analysis

Precentrifugation

before centrifuge

QNS

Quality not sufficient

RAM

random access memory, main memory,temporary storage for data that will be lost when computer is shut off

RFID

radio frequency identification

RNA

ribonucleic acid, must be processed immediately or stored at 4 degrees C for only 48 hrs

ROM

read only memory,installed by manufacturer,permanent

Software

programming, coded instructions,designed to operate computer

Storage

preserving information

Terminal

computer screen/keyboard combination

USB drive

universal serial bus

What levels are tested for Electrolyte Testing?

< Sodium(Na+), Potassium(K+) >Bicarbonate(HO3-),and Ionized Calcium(iCa2+)

What are Types of Communication Barriers?

Gender, Age, Language, Emotions, Culture, and Physical Issues

What Tubes produce Serum?

Red, Gold, Tiger Top/ SST

Venipuncture Order of Draw<Tube Colors and Additive>

1.Sterile 2.Light Blue(Citrate)3.Red(serum) 4.Tiger(serum)5.Gold(serum) 6.Green(Heparin)7.Lavender(EDTA)8.Grey(Oxalate)9.Royal Blue(EDTA)10.Yellow(ACD)

Capillary Order of Draw <tube color and Additive>

1.White (Blood Gas)2.Lavender (EDTA)3.Green(Heparin)4.Grey (Oxalate)5.Red (Serum)6. Black ( NewBorn Screens)<Card>

What does a Red top Tube Contain/Do?

Clot Activator/ Clots Blood

What is the "rule" for tourniquet use before and during blood draw?

1min. before and 2min while obtaining blood sample

What "tubes" are used for Blood Culture?

Anaerobic and Aerobic Sample Bottles

What is the proper chain of events for a GTT?

Fasting Blood Sugar<Within Range>Glucola drink Finish within 5minsthen test every 1/2,1,2,3 hours from time of finished drink

What does CLIA '88 stand for?

Clinical Laboratory Improvement Amendments of 1988

What does CLSI stand for?

Clinical & Laboratory Standards Institute

What does OSHA stand for?

Occupational Safety & Health Administration

What ID is required for Outpatient Testing?Inpatient?

Outpatient: Name & DOBInpatient: ID band matches Requistion

Grey Top Additive(s) and Department? Yield?

Sodium FluoridePotassium OxalateChemistryPlasma

Light Blue Top Additive(s) and Department? Yield?

Sodium CitrateCoagulationPlasma

Red Top,Tiger(SST),Gold Additive(s) and Department? Yield?

NoneClot Activator and gel separatorChemistrySerum

Green Top Additive(s) and Department? Yield?

Lithium Heparin (Chemistry)Sodium Heparin (Send Out Lab)Plasma

Why do you wipe away the first drop of blood in capillary testing?

To remove tissue "juices" and possible alcohol residue

What Additives Yield Plasma?

EDTALithium/Sodium HaparinPotassium OxalateSodium Citrate

What equipment is used to obtain blood in Venipuncture?

TourniquetGauzeAlcohol SwabCollection Device ( Multi draw needle, ETS holder, Butterfly,Ect..)Evacuated TubeCo-banGloves

What equipment is used to obtain blood in Capillary puncture?

GauzeAlcohol SwabMicrotainerLancetBand-aidGloves

A Jaundiced appearance of a patient means?

Liver Inflammation with High levels of Bilirubin

What causes Hemoconcentration?

Dehydration (decrease in fluid content of the blood, with an increase in non-filterable large molecule,or protein-based blood components such as RBC)

What are some reasons you might have Hemolysis?

drawing blood through a HematomaSlow Blood FlowRough handling of specimenfailure to wipe away first drop of blood(capillary) which could contain alcohol residuePage 284 Box 9-3 for a complete list

What is considered the most dangerous to a patient?

Not properly Labeling the tubes

What does Hemolysis Mean?

Red blood cells are destroyed

Amonia Test (tube,additive,Lab, Special handling)

Green,Lithium Heparin ,Chemistry, Ice Slurry

Lactate Test (tube,additive,Lab, Special handling)

Gray, Potassium OxalateChemistry, NO Tourniquet

Heavy Metal Testing (tube,additive,Lab)

Royal Blue, EDTASend Out Lab

If you get Serum in your eye what procedures do you take?

Eye wash for 15min, Fill out an Incident report and go to Employee Health

What are the Main components of the Circulatory System?

Heart, Vascular System, Blood,Hemostasis &Coagulation,Lymphatic systems

What are the Functions of the Circulatory System?

Carries oxygen and food to cells and carries carbon dioxide away, aids in coagulation,Runs the heart(cardiac cycle), circulates blood through out the body, removes micro-organisms and impurities

What are the 3 layers of the heart?

Epicardiummyocardiumendocardium

What Color tube and Lab for Liver enzymes?

Red,Green,Gold,Tiger(SST)Chemistry

What creates RBC?

Bone marrow

what are the 3 phases of Coagulation?

1.Initiation2.Amplification3.Propagation

What phase is Factor V(5)?

Initiation & Amplification

The Joint Commisions Sentinel Event QI program policy is designed to do

-Identify unfavorable events for immediate investigation-improve the safety of patients in healthcare institutions-prevent unfavorable events from happening again

What is NOT a phlebotomist's duty

Starting intravenous lines (IV)

Malpractice is a claim of

Improper treatment

Blood Bank

Immunohematology